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色谱  2002 

Determination of Benzoyl Peroxide in Wheat Flour by High Performance Liquid Chromatography
高效液相法测定面粉中的过氧化苯甲酰

Keywords: high performance liquid chromatography,benzoyl peroxide,wheat flour
高效液相色谱法
,测定,面粉,过氧化苯甲酰,改良剂,食品添加剂

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Abstract:

An improved method for the determination of benzoyl peroxide in wheat flour is reported. Benzoyl peroxide was extracted with petroleum ether, and then analysed by reversed-phase high performance liquid chromatography on a Shim-Pack VP-ODS column (0.46 cm i.d. x 15 cm, 5 microm - 6 microm) using a mobile phase of acetonitrile-0.3% H3PO4 aqueous solution (4:1 in volume ratio) with a flow rate of 1.0 mL/min, and UV-236 nm detection. A linear correlation was observed between 0.002 g/L- 0.012 g/L of benzoyl peroxide. The detection limit was 0.005 g/kg. This proposed method without redox reaction is superior to colorimetric determination. This method is characterized with simplicity, convenience, reproducibility and accuracy.

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