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色谱 1999
Study on Qualitative Analysis Method of High Performance Liquid Chromatography by Simultaneous Multi-Wavelength Detection
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Abstract:
In this paper, we introduce an HPLC qualitative method , that is, multiwavelength detection qualitative analysis method. According to Beer's law, for a certain compounds the ratio of peak areas at different wavelengths is a constant. Comparing the ratio of peak areas of a component at different wavelengths with that of the standard by means of simultaneous multi-wavelength detection, we established a convenient qualitative method. This method has been applied successfully to qualitative analysis of 18 aromatic ammes in the prohibited azo dyes.
