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色谱  2011 

Simultaneous determination of four mercapturic acids in human urine using solid phase extraction and liquid chromatography-tandem mass spectrometry
固相萃取-高效液相色谱-串联质谱法同时测定尿液中的4种巯基尿酸

Keywords: liquid chromatography-tandem mass spectrometry (LC-MS/MS),solid phase extraction (SPE),mercapturic acids,urine
液相色谱-串联质谱
,固相萃取,巯基尿酸,尿液

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Abstract:

A method was developed for the simultaneous extraction and determination of four mercapturic acids(MAs),N-acetyl-S-(3,4-dihydroxybutyl)-L-cysteine(DHBMA),N-acetyl-S-(3-hydroxypropyl)-L-cysteine(3-HPMA),N-acetyl-S-(2-carboxyethyl)-L-cysteine(CEMA) and S-phenylmercapturic acid(SPMA),in human urine using solid phase extraction and high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Frozen urine samples were thawed at room temperature,and centrifuged to remove any settled precipitate.The...

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