Analysis of the Composition in Fructooligosaccharide by High Performance Liquid Chromatography
高效液相色谱法测定低聚果糖的组分

In this paper, an analytical method for determination of fructooligosaccharide by high performance liquid chromatography(HPLC) is presented. The HPLC conditions are mu-Bondapak C18 column, 300 mm x 3.9 mm i.d., water as mobile phase and refractive index detector. Monosaccharide, disaccharide, trisaccharide, tetrasaccharide, pentasaccharide, hexasaccharide etc. can be simultaneously determined in a single injection. The method has been used to determine the fructooligosaccharides in "Boai biogen". The results are satisfactory. The CVs were 0.55%-1.1% and correlation coefficients were 0.9992-1.000. The lowest detectable limit for fructooligosaccharide was in microgram level.