Determination of mannitol,monosaccharides and lactulose in mouse urine by high performance anion exchange chromatography coupled with pulsed amperometric detection
高效阴离子交换色谱-脉冲安培法检测小鼠尿液中的甘露醇、单糖和乳果糖

A method of high performance anion exchange chromatography (HPAEC) coupled with pulsed amperometric detection (PAD) was established for the determination of mannitol, monosaccharides (galactose, glucose, mannose and fructose) and lactulose in mouse urine. The samples were first centrifuged, and then filtered through molecular film to eliminate the proteins. The analysis was performed on a CarboPacTM PA1 column using gradient elution with water and 250 mmol/L sodium hydroxide as the mobile phase. All of the six carbohydrates had good linear relationships (0.988≤r2≤0.999) in the range of 0.1~5.0 mg/L. The recoveries were between 95.5% and 104.2% and the limits of detection were between 0.0013 and 0.004 8 mg/L. The method is accurate, fast and simple. It can be used to analyse six carbohydrates simultaneously and track the metabolic relationships among mannitol, monosaccharides and lactulose during the metabolism of the carbohydrates.