Analysis of paracetamol, caffeine and chlorphenamine maleate in suxiaoshangfeng capsules by capillary gas chromatography]
毛细管气相色谱法测定速效伤风胶囊中对乙酰氨基酚、咖啡因、马来酸氯苯那敏的含量

A simple and rapid method for simultaneous determination of paracetamol, caffeine and chlorphenamine maleate in Suxiaoshangfeng capsule by capillary gas chromatography with n-hexadecane as internal standard has been reported. In the capsule, the three components can be separated on a 5% Ph-Me-Silicone column (2.65 microns x 0.53 mm x 10 m), with programmed column temperature from 180 degrees C to 230 degrees C at 8 degrees C/min, hold 6 min and then 230 degrees C to 260 degrees C at 10 degrees C/min, hold 10 min. Both the injector and FID detetor temperatures were 280 degrees C. N2 flow rate was 3.5 mL/min and H2 35 mL/min. The injection volume was 1 uL with splitting ratio of 1/25. These components were identified by their retention times and quantitatively determined by their peak areas. The calibration curves were, r = 0.9996 for paracetamol, r = 0.9984 for caffeine and r = 0.9996 for chlorphenamine maleate. The linear ranges were 4.0-20 g/L, 0.15-0.75 g/L and 0.080-0.40 g/L, respectively. The average recoveries were 99.62% (RSD = 0.40%, n = 5), 96.46%. (RSD = 1.32%, n = 5) and 98.55% (RSD = 0.65%, n = 5), respectively.