Determination of Nanchangmycin and Meilingmycinby High Performance Liquid Chromatography
高效液相法同时检测南昌霉素和梅岭霉素

A high performance liquid chromatographic method had been established for the separation and determination of two antibiotics produced by Streptomyces nanchangensis, polyether nanchangmycin and 16-membered macrolide meilingmycin. The latter is composed of several components. The operating conditions were Waters XTerra RP18 column (3.9 mm i.d. x 150 mm, 5 microns) at 25 degrees C, mobile phase linear gradient elution of acetonitrile-water with 57:43 (volume ratio) during 0 min-30 min, 70:30 during 30 min-32 min, 80:20 during 32 min-34 min, 90:10 during 34 min-50 min at a flow rate of 0.8 mL/min, and photodiode array detector at 234 nm. This method is accurate, rapid and simple, and can be used for the integrated analysis of the two different natural compounds, polyethers and macrolides.