Isolation and Purification of Puerarin from Puerarin Extractive by Chelate Complex Chromatography
配位色谱法从葛根素浸膏中分离纯化葛根素

Puerarin, an isoflavone compound, is an important ingredient in traditional Chinese medicine, which has the good medical effects on hypertension and angina. A new type of separation and purification method for isolating puerarin was developed using chelate complex chromatography. The stationary phase was prepared with silica gel containing 7% of Cu(OAc)2 in the chloroform. After the chloroform was evaporated, it was used as chelate complex chromatographic stationary phase to purify puerarin extractive. Pure puerarin was obtained when a mixture of chloroform and methanol (10:1, v/v) was used as eluent. In comparison with a common silica gel column, the chelate complex chromatographic column was more convenient and efficient. The purity and recovery of puerarin, and column capacity were improved 11%, 12% and 200%, respectively. Under the optimized experimental conditions, the purity of puerarin could be as high as with a recovery of 92%.