Application of High Performance Liquid Chromatography to the Study on the Production of Maltooligosaccharide by Synergistic Action of Two-Enzymes
高效液相色谱法在双酶协同作用酶解淀粉制取麦芽低聚糖工艺研究中的应用

This paper describes the application of high performance liquid chromatography to the study on the production of maltooligosaccharide by synergistic action of two-enzymes. Seven sugars in products were separated on a column of Nucleosil C18 with water as the mobile phase and determined refractometrically. Meanwhile, for the preparation of maltooligosaccharide, the effect of supplementing rate of the two enzymes on the content of maltooligosaccharide in products is examined in this paper. The optimun technolgical conditions were obtained by using the orthogonal design and the amount of the two enzymes added was determined. Thus more than 70% of M3-M6 maltooligosaccharide in product was obtained.