Study on Homogeneity of Enzymatic Degradation of Chitosan as Biomaterials by Gel Permeation Chromatography
凝胶渗透色谱法研究壳聚糖生物材料酶降解过程的均匀性

Chitosan is an important biomedical material,and its degree of deacetylation is a main parameter of its biodegradation.Gel permeation chromatography was used to investigate the lysozymic degradation of two types of chitosan samples(A and B) with similar degree of deacetylation and relative molecular mass but with different distributions of two units of N-acetyl-D-glucosamine and D-glucosamine.Weight average relative molecular mass,polydispersity and gel permeation chromatograms during the degradation process were obtained.It was found that chitosan sample A with random distribution of the two units underwent a homogeneous degradation process while chitosan sample B with block distribution underwent a heterogeneous degradation process.The results suggest that the homogeneity of the degradation of chitosan materials by lysozyme depends on the distribution type of the two units,which can help to design chitosan-based biomedical devices.