Determination of Glycyrrhetic Acid in Toothpaste by Reversed\| Phase High Performance Liquid Chromatography (RP\|HPLC)
反相高效液相色谱法测定牙膏中的甘草次酸

A method for determining glycyrrhetic acid in toothpaste by RP-HPLC is described. The operating conditions were YWG-C18 column (4.0 mm i.d. x 250 mm, 10 microns) at room temperature, V(methanol):V(0.01 mol/L KH2PO4) = 85:15 (pH 3.0) as mobile phase with flow rate of 1.0 mL/min and UV detection at 254 nm. Glycyrrhetic acid in toothpaste samples were extracted with methanol. The average recoveries of glycyrrhetic acid were 99.61%-101.67%. The relative standard deviations were 1.85%-3.16%. The method is simple, rapid and accurate.