Determination of three adrenergic drugs using capillary electrophoresis with amperometric detection
毛细管电泳-安培检测法测定3种拟肾上腺素药物

A method for the determination of three adrenergic drugs, including phenylephrine hydrochloride (PHE), metaraminol bitartrate (MR) and isoprenaline hydrochloride (IP), was developed using capillary electrophoresis with amperometric detection. The detection potential of working electrode was 0.950 V versus the reference electrode of Ag/AgCl. At the applied voltage of 18 kV, the three analytes were completely separated within 18 min in 50 mmol/L borate buffer (pH 10.00) with the injection time of 10 s. Good linear relationships were obtained for all the three analytes in the range of 2~100 μmol/L. The detection limits for PHE, MR and IP were 0.8, 0.8 and 1.0 μmol/L, respectively. The proposed method was applied to the analysis of some injection drugs, and the results were satisfactory.