The Study of Ethanol Water Used as Reversed Phase High Performance Liquid Chromatographic Mobile Phase
乙醇-水作为反相高效液相色谱流动相的研究

The viscosity of ethanol water at various temperatures was determined.The physicochemical properties and characters of the solvents used as the mobile phase of reversed phase high performance liquid chromatography(RP HPLC) were compared between methanol and ethanol.The components of several Chinese medicines have been determined by RP HPLC with ethanol water used as mobile phase , and the results were compared with those obtained with methanol water or acetonitrile water. It showed that ethanol can be used as RP HPLC mobile phase by increasing the column temperature moderately .