Separation and determination of tyramine by reversed-phase high performance liquid chromatography]
反相高效液相色谱法分离测定酪胺

The method for separation and determination of tyramine by reversed-phase high performance liquid chromatography has been established. The effects of mobile phase on mechanism of separation based on the ion interaction are discussed. Using C8 alkyl bonded phase and 40% methanol-water solution containing Trisperchlorate as pH buffer (adjusted pH to 7.9) and ion interaction reagent and applying p-toluene sulfonamide as internal standard substance, the tyramine obtained from decarboxylation of p-tyrosine was determined. The RSD of determination was 0.66%(n = 11). The recoveries of sample were between 99.33% and 100.38%.