The Factors Affecting the Symmetry of Chromatographic Peak in Liquid Preparative Adsorption Chromatography
液相制备吸附色谱流出峰对称性的若干影响因素

The symmetry of chromatographic elution curve has a direct influence on the separation efficiency and recovery yield. Whether the standard chromatographic peak is symmetric or not has been a disputed issue based on the plate theory or the diffusion theory in recent years. In the present paper the researches were done on the basis of continuity equation of chromatographic separation and the rate theory of mass transfer in order to show the relation between the shape of chromatographic peak and the thermodynamic and dynamic parameters, as well as operation conditions quantitatively. The non linear chromatographic separation model was established and solved by using the characteristic curve method. The factors affecting the symmetry of chromatographic curve were analyzed through computer simulation. Research results indicated that the normal chromatographic elution curve is asymmetric and the asymmetric degree of elution curves increases with the increase in the non linear degree of the system, the mass transfer resistance, the feed volume or concentration and the fluid velocity and with the decrease in the adsorption capacity of adsorbent. These results are useful to improve the separation efficiency and recovery yield.