Determination of Fatty Acids in Nematode by Gas Chromatography
气相色谱法测定线虫中的脂肪酸含量

ematode is a small organism and the traditional purification method needs a lot of nematodes , but it is difficult for us to foster many nematodes.The fatty acids in nematode were esterified directly in our analytical method,so that,the error is reduced the and sensitivity is improved. The results showed that thisimproved analysis method could be used for rapid and satisfactory determination of the fatty acid change during storagr of this group of nematodes.The fatty acids in the nematode were analysed by gas chromatography. Seven kinds of main saturatd and unsaturated fatty acids with 16 to 20 carbon atoms were separated on the FFAP capillary column.The results of quantitative and qualitative analysis are satisfactory.