Sensitive Determination of Amino Acid Composition of MotherLiquor Prepared by Hydrolyzing PigHair with Reversed-Phase High Performance Liquid Chromatography(HPLC)
猪毛水解母液中氨基酸的反相高效液相色谱分析

new method for separation and quantification of amino acids by pre-column derivatization withphenylisothiocyanate(PITC ) and reversed-phase HPLC is described. Seventeen amino acids were separatedin 30 min at room temperature.The method has been successfully applied to the determination of amino acidcomposition in the solution which was prepared by hydrolyzing pig hair and extration of cystine.