Comparison in Hydrophobic Selectivity for Two Types of Reversed-Phase High Performance Liquid Chromatography Packing Material
两种新型反相高效液相色谱填料疏水选择性能的比较

Two types of novel reversed-phase packing materials were made by adding TiO_2/SiO_2(or ZrO_2/SiO_2) and octadecyltrichlorosilane into toluene with stirring and refluxing for 36 h.TiO_2/SiO_2 and ZrO_2/SiO_2 particles were prepared by a layer-by-layer self-assemble technique and consist of micrometer-sized silica spheres as cores and nanometer-sized zirconia or titania as surface coating.The carbon loading of C_18-bonded TiO_2/SiO_2 was 11.51% and that of C_18-bonded ZrO_2/SiO_2 was 9.62%.The hydrophobic selectivity and sensitivity were studied respectively and compared in details;the results showed that the packing supports both acted as true reversed chromatographic stationary phase with similar hydrophobic selectivity.The chromatographic behaviors of the two types of novel reversed-phase packing material were evaluated with nine aromatic compounds.The chromatographic studies showed that either of the packing materials would be a good choice to separate aromatic compounds,however,the selectivity of C_18-bonded TiO_2/SiO_2 was a little better than that of C_18-bonded ZrO_2/SiO_2.