High Performance Gel Chromatographic Behaviour of Sodium Alginate and Determination of Its Molecular Weight Parameters
褐藻酸钠的高效凝胶色谱行为及其分子量参数测定

The molecular weight parameters of sodium alginate were determined by high performance gel chromatography with Waters Bondagel E-High A column. The differences in high performance gel chromatographic behaviours between the sodium alginate and the non-polar standard Dextran T500 were compared. The influences of the sample concentration and the sample volume on the results were discussed. The high performance gel chromatographic behaviour of sodium alginate is different from that of Dextran T500,and is also different from those on Sepharose or Sephadex columns. The peak shifted toward full osmotic side under in crease of ionic strength. The elution volumn didn't tend toward stable, even though at the high concentration of 0. 5mol/L NaCl- A change of the sample concentration at stable sample volume or the sample volume at stable sample concentration would cause tailing peaks. It was showed that the samp1e size of sodium alginate would be less than 25pg, and the relative standard deviations of Mn Mw and d were 0. 95%,4.04% and 4. 70%.