Determination of the Soluble Non Starch Polysaccharides in Rice and Wheat Bran by Gas Chromatography
稻子、小麦麸皮中可溶性非淀粉多糖的气相色谱法测定

Cereal polysaccharides can be broadly classified into two distinct and chemically well-defined types. They are the storage polysaccharides (alpha-glucan) and the structural polysaccharides starch (beta-glucan) which are usually called non-starch polysaccharides (NSP). The determination of soluble non-starch polysaccharides in rice and wheat bran by gas chromatography has been developed. The free sugars in the sample were extracted with 80% ethanol. The residue was hydrolyzed in an acetic acid buffer solution (pH 5.0) in the presence of amylase and amyloglucosidase to remove starch. The soluble NSP obtained was further hydrolyzed in the acidic condition to produce the corresponding monosaccharides which were derivatized to form alditol acetates for GC analysis with allose as the internal standard. The GC conditions were OV-1701 column (25 m x 0.3 mm) with temperature program from 195 degrees C to 225 degrees C and FID.