Immobilization of N Acetyl 1,6 Diaminohexane(NADAH) on Diol Silica and Study of Mechanism of NADAH and Hexamethylene Bisacetamide(HMBA)Inducing Tumor Cells Differentiation
N-乙酰-1,6-二氨基己烷的固定化及其诱导肿瘤细胞分化机理的研究

A method for separating NADAH and its immobilization onto diol-silica have been developed. HMBA and its meabolite NADAH were used as the inducers for many types of tumor cells' differentiation. The inducing mechanism of HMBA is not clear yet. Experiments show that HMBA and NADAH have a relatively strong hydrophobic reaction and hardly ion-exchange with some DNA and some proteins of the cytosolic fraction of HL-60 human acute progranulocytic leukemia cells, BIU-87 human bladder carcinoma cells and the human erythrocyte membrane, when utilizing immobilization of NADAH. The retention time of the proteins and DNA was longer than that of the phosphatides. These results show that the adsorption of HMBA and NADAH with some proteins and DNA is higher than that with the phosphatides. The expected receptor binding biospecifically with HMBA has not been found.