A Novel Method for Preparation of C18 Ester-BondRP-HPLC Packing
一种新型C18酯型反相高效液相色谱填料的制备

RP-HPLC packings have found widespread application in the fields of biology, chemistry and pharmaceutical industry, etc. C18-bonded phase is one of the most important packing. In order to avoid using toxic and unstable chloro-containing silane as derivatization reagent, a new practical and economic procedure had been developed to prepare RP packing. Octadecyl group was bound onto YQG80, 5 microns silica (Tsingdao Oceanic Chemical Factory, particle size d.p. 5 microns) with gamma-glycidoxypropyltrimethoxysilane as coupling agent. Elemental analysis and chromatographic evaluation confirmed the feasibility of the procedure and RP behavior of the packing. After eluting with 500 column volume of mobile phase at different pH (2, 5 and 8), the column still showed good performance (> 64,000 theoretical plates/m) and stability.