A Gas Chromatographic Method for Determination of Butyraldehyde as a Product of Butanol Biotransformation in Rat Liver
气相色谱法测定大鼠肝微粒体中丁醇的生物转化产物丁醛的含量

Analysis of butyraldehyde obtained by the interaction of butanol with cytochrome P450 II E1 in rat liver microsomes prepared by centrifugation and with the NADPH added is described in this paper. The biotransformation rate of butanol into butyraldehyde can be used as an index for the assessment of the enzyme activity of cytochrome P450 II E1. A headspace gas chromatograghic method to determine the butyraldehyde has been developed. The detection limit and CV of this method for butyraldehyde in microsomes were 0.7 micromol/L and 8.1%-9.3% respectively. The recovery was 85.3%. The results show that this is a rapid and sensitive method with less interferences and fairly good precision. The method developed has made a reliable analytical methodological foundation for the assessment of the enzyme activity of cytochrome P450 II E1.