全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
色谱  2001 

A New Method for Predicting Retention Time of Solutes Under Linear Gradient Elution Conditions in RP-HPLC
反相高效液相色谱中流动相线性梯度洗脱条件下溶质保留时间预测的新方法

Keywords: high performance liquid chromatography,linear gradient,retention time,prediction
反相高效液相色谱法
,保留时间,预测,反相,流动相,溶质,线性梯度洗脱

Full-Text   Cite this paper   Add to My Lib

Abstract:

A new method for predicting the retention time of solutes under linear gradient elution has been established In this method, the migration distance of solutes in column and the mobile phase gradient were modified simultaneously according to the distribution of mobile phase in column The prediction results of retention time of both weakly and strongly retentive solutes were accurate in different instrumental systems using this method In order to test and verify this method, the retention time of fifteen amino acids and eight homologous compounds of benzene were predicted The predicted results were compared with those predicted by the method in common use The relative average errors between predicted and experimental values were 3 70% and 4 90% for the weakly retentive solutes, far smaller than those obtained by the reference method (23 61% and 31 16%) For the strongly retentive solutes, the relative average errors were 0 21% and 6 01% respectively, slightly smaller than those obtained by the reference method (0 81% and 6 69%) It is seen that this new method has many advantages

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133