An Equation Describing the Relationship Between Injected Amount of Solutes and Their Retention Values in Liquid Chromatography
一个描述液相色谱体系中溶质进样量与保留值关系的方程

n equation describing the reistionship between the injected amount of solutes and their retention values in liquid chromatography was theoretically presented by considering the interaction between the solute molecules and the solvent molecules on the surface of stotionary phase.It can be proven that when the injected amount of solutes separately approach to infinity or to zero, their retention values separately approach to zero or to a constant depending on the chromatographic system, and when the injected amount of solute increases from zero to infinity, the retention value will constantly decrease. By using the linear form of this equation, two important parameters describing the characteristics of the chromatographic system can be determined. One is the thermodynamic equilibrium constant K for solute absorption on the surface of the stationary phase and another is the total mean active sites hypothetically distributed on the surface of stationary phase. Some proteins were used to test this equation in hydrophobic interaction chromatography, and it was found that the predicted retention values by using this equation are very consistent with the experimental ones, the deviations between them are less than 5%.