Screening of Steroidal Saponins from the Bulbs of Lilium Brownii var.colchesteri by Combination of High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry and Electron Impact Mass Spectrometry
液相色谱-电喷雾电离质谱与电子轰击质谱联用筛选百合中的甾体皂甙

With the combination of high performance liquid chromatography electrospray ionization mass spectrometry (HPLC/ESI MS) and electron impact mass spectrometry (EI MS), two steroidal saponins, one compound containing three glycosyls and tigogenin and the other one containing three glycosyls and diosgenin, from the bulbs of Lilium brownii var colchesteri in China have been screened In the method, on line HPLC/ESI MS allows us to obtain rapidly useful information about the molecular weight and the glycosyl chain of glycoside without the necessity of isolating individual compounds, but little information about steroidal sapogenins Just with 1 mg to 2 mg of pure sample, off line EI MS allows us to acquire useful information about a steroidal sapogenin of saponins, but it is difficult to obtain the molecular ion peak The combination of HPLC/ESI MS and EI MS is well suitable for rapidly screening steroidal saponins from plants