Simultaneous Separation of the Metabolins of Amino Acids,Organic Acids and Nucleosides in Clinical Chemistry by Two-Dimensional Thin-Layer Chromatography
临床化学上氨基酸、有机酸及核苷类的二维薄层色谱多相分离

Over 30 amino acids, 40 organic acids and 20 nucleosides and related compounds have been simultaneously separated by two-dimensional thin layer chromatography on a plate. The method of solvent extraction for the non-volatile organic acids in urine was improved. And the location of the separated compound spots was performed with a multiple procedures: Viewing the absorption spots in UV light (254nm)---colouration in acridine reagents for most organic acids and ninhydrin-cadmium acetate for amino acids.