Trace-Analysis of Amino Sugars and NeutralSaccharides by High Performance Capillary Electrophoresis(HPCE)and High Performance Liquid Chromatography(HPLC)
痕量氨基糖和中性单糖的高效毛细管电泳及液相色谱分析

ecause of the low UV absorbance of carbohydrates, the active fluorescence and ultraviolet labellingreagents,9-fluorenylmethoxycarbonyl(FMOC ) hydrazine and 9-fluorenylmethoxycarbonyl chloride,havebeen utilized as derivatization reagents for monosaccharides in the present work.HPCE and HPLC coupledwith ultraviolet and fluorescence detector was studied as a means of separation and determination of sugars.The results show that monosaccharides derivatized with FMOC-hydrazine and FMOC-Cl can be determinedwith high sensitivity at fmol level. The HPCE separation conditions of monosaccharides were studied in de-tails.