Effect of Some Factors on Oxalate Content in Buckwheat Seedlings
不同因子对荞麦中草酸含量的影响

Buckwheat (Fagopyrum esculentum Moench) seedlings in Hoagland solution were fed with different substances, and oxalate contents in the roots and leaves were determined after 36 hours. The results showed that ascorbate and its precursors as well as isocitrate decreased to some extent the oxalate content in roots and leaves, whereas glycolate and glyoxylate significantly increased oxalate content, suggesting that oxalate in the leaves was synthesized mainly from glycolate pathway. Oxalate content in leaves of buckwheat seedlings cultured under hydroponic condition with ammonium nitrogen or urea as the only nitrogen source was much lower than that with nitrate nitrogen. The same result was observed under hydroponic condition with nitrate nitrogen in which glutamate or serine was added. It was shown that different nitrogen sources affecting oxalate accumulation in buckwheat leaves were closely related to glycolic acid metabolism.