The Induction of Bulbils of Dioscorea zingiberensis
盾叶薯蓣试管株芽的诱导

An in vitro protocol for rapid propagation of medicinal plant Dioscorea zingiberensis C. H. Wright was optimized. Nodal segments with buds cultured obtained from vessels were used as explants which were then cultured on bulbil induction medium. The explants started to appear emergence from basal segments after 15 days. Three to five bulbils with roots developed from each segment were obtained after another 15 days. The optimal culture conditions for bulbil formation were 262 at 1 500-2 000 lx illuminated for 14 hours per day on MS medium supplemented with 4.0 mg L-1 BA and 1.0 mg L-1 IBA in addition of 6%-9% sucrose, 0.5% active charcoal, and 7% agar. The bulbil induction rate was 100%, and the plantlet survival rate being above 90%.