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Expression of Human Lactoferrin in Transgenic Potato Tubers
人乳铁蛋白在转基因马铃薯块茎中的表达

Keywords: Lactoferrin,Transgenic,Potato,Tuber,Expression
乳铁蛋白
,致转基因,马铃薯,块茎,表达

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Abstract:

Human lactoferrin (hLF) is a member of non-specific immune system, it plays many roles, such as resistances against bacteria, fungi and viruses, etc. In this paper, hLF cDNA directed by the tuber specific promoter of class I patatin gene of potato (Solanum tuberosum L.) were transferred into potato mediated by Agrobacterium tumefiens transformation. hLF transgenic potato plants were confirmed by PCR detection, further RT-PCR showed that hLF RNAs were expressed in transgenic plants. hLF activities were detected by ELISA and Western blot analysis in hLF transgenic potato tubers.

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