NUCLEAR GENOMIC HOMOLOGOUS SEQUENCE OF CHLOROPLAST psbA GENE IN RICE (ORYZA SATIVA L.)
水稻(Oryza sativa L.)核基因组存在叶绿体psbA基因的同源片段

Sequence comparison showed the high homology between rice repetitive DNAsequence pRRD9 and chloroplast gene psbA encoding QB protein. Southern hybridizationsof rice chloroplast and nuclear DNA with the two subcloned segments from pRRD9,pRRD9R and pRRD9L, revealed that psbA gene sequence was transferred from chloroplastto the nuclear genome in rice at a certain evolution stage. Significant differences occurredbetween the two segments in the ev0lution of rice variation. Southern analysis of wild andcultivated rices with the segments showed that the cultivated Asian rice had closer relativeof the wild rice with AA genome type. In addition, specific hybridization patterns werealso detected in some wild rices. These findings suggest that pRRD9 may be a goodmolecular probe for the study of the evolution of rice.