Cloning and Sequence Analysis of Expansin gene from Cambium of Anthocephalus chinensis
团花树形成层扩展蛋白基因cDNA的克隆和序列分析

Using the cDNA from the branch cambium of Anthocephalus chinensis as the template, the degenerate primers by reference to the α-Expansin gene (EXPA) sequences in other plants were constructed for RT-PCR. Taking the production of the amplification as the starting information, The full-length cDNA of expansin gene was obtained by RACE, named as AcEXPl. It was 979 bp in length (GenBank accession No. FJ417847) with 777 bp ORF, encoding 258 amino acid, which had the typical conserved sequence and domain of Expansin. There were 85%, 85%, 84%, 83% homobgy with Popuha tomentosa, Populus tremula P. tremubides, Prunus cerasus, Petunia hybrida, respectively. It provided the foundation for studying the relationships between EXP gene and growth rate, wood quality of the xylem of Anthocephalus chinensis.