EFFECTS OF SEVERAL AUXINS ON SOMATICEMBRYOGENESIS AND PLANT REGENERATION IN CASSAVA
几种生长素对木薯体细胞胚发生和植株再生的作用

Primary somatic embryogenesis could be directly induced from immature leaves ofcassava (Manihot esculenta Crantz) on the MS solidified medium supplemented with higherconcentration of 2,4-D (1 - 16 mg L-1) or NAA (40 mg L-1), but IBA and IAA (40 mg L-1)could not induce it. When the somatic embryo fragments were used as explants, the needof auxin activity and concentration for the induction of secondary somatic embryogenesisdecreased. The decrease of the concentration or activity of the auxins in the induction mediacould enhance somatic embryogenesis and root formation, and result in the increase ofplant regeneration frequency. It was observed that somatic embryos could not developshoot meristem on the induction medium containing 4 mg L-1 2,4-D. The experimentsshowed that the auxins stimulated the induction of cassava somatic embryogenesis butinhibited the development of somatic embryos and decrease plant regeneration in cassava.