CHANGES IN GROWTH PARAMETERS OF EMBRYOGENIC CELL SUSPENSION CULTURES OF LOBLOLLY PINE (PINUS TEEDA)
火炬松胚性细胞悬浮培养物的生长参数变化

Embryogenic calli derived from mature zygotic embryos of loblolly pine (Pinus taeda L.) of three genotypes from Hunan Province, viz. Shaoyanghigh-2, Yongshunmiddle-1 and Yongshunmiddle-3 were used in embryogenic cell suspension culture. Parameters in the growth of cultures, including fresh weight, dry weight, packed cell volume, somatic embryo number, as well as pH, conditivity, and sucrose concentration of the culture medium were measured during culture period. The results showed that, during culture circle, the decrease in pH, conductivity, and sucrose concentration in the medium was shown to be related to the increase of fresh and dry masses, packed cell volume, and somatic embryo number of cultures. pH, conductivity, and sucrose concentration at the 18th to 21st days of culture were at lowest level, while embryo number and packed cell volume were at highest.