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OALib Journal期刊
ISSN: 2333-9721
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Cloning of PsbS Gene from Moso Bamboo (Phyllostachys edulis) and Its Characterization of Prokaryotic Expression
毛竹PsbS基因的克隆及其原核表达特征研究

Keywords: Phyllostachys edulis,PsbS gene,Clone,Construction of expression vector,Prokaryotic expression,Temperature
毛竹
,PsbS基因,克隆,载体构建,原核表达,温度

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Abstract:

PsbS protein plays an important role in non-photochemical quenching(NPQ) in plants.A PsbS gene was cloned from Moso bamboo(Phyllostachys edulis) leaves by RT-PCR method,which named PePsbS(GenBank No.FJ600727) with 810 bp in length,and encoded a polypeptide with 269 amino acids.Homology analysis showed that the deduced PsbS was highly homologous to those from other monocotyledon plants.The protein structure analysis indicated that PePsbS consists of transit peptide and mature protein including four transmemb...

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