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热带亚热带植物学报 2008
Plant Regeneration in vitro of Cuscuta chinensis Lam.
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Abstract:
An efficient method of callus induction and plant regeneration of Cuscuta chinensis was established. The seedlings germinated for 3~5 d and about 3~5 cm length were selected as explants, and then divided into three parts, upper, middle and lower. Callus were inducted from upper or middle parts of seedlings on a modified Murashige and Skoog (MMS) medium supplemented with 1 mg L-1 NAA and 1 mg L-1 BA. The calli have been subcultured on such medium for over a year. Shoot regeneration from callus was achieved on MMS medium containing 1 mg L-1 BA and could obtain 2.8 shoots per callus.
