SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM AXES OF PEANUT EMBRYOS
花生体细胞胚的诱导及其植株再生

Somatic embryos could be induced from axes of mature peanut embryos culturedon hasal MS medium containing high concentration of 2,4-D (40 mg L~-1) with 0.5 mg L~-1kinetin, 30% sucrose and 0.9% agar for 30 days, the induction rate being 100 percent,and the mean embryo number per explant reaching 11.58. Lower concentrations of 2,4-D(10-20 mg L~-1) were suitable for somatic embroid differentiation in subculture. However,10 mg L~-1 2,4-D was the optimum concentration for inducing somatic embryos both inprimary culture and in subculture by immature embryo axes. Somatic embryos could easilygerminate on MS medium with 5- 10 mg L~-1 BA. The plantlets were obtained afterthe shoots were transferred to root-inducing medium, MS 2 mg L~-1 NAA.