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热带亚热带植物学报 2007
Genetic Diversity of Chinese Main Banana Cultivars (Musa spp.) and Introduced Accessions from INIBAP Using Simple Sequence Repeats (SSRs)
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Abstract:
Microsatellites were used to investigate the genetic diversity and relationships among 14 Chinese main banana cultivars(Musa spp.) and 33 introduced accessions from International Network for the Improvement of Banana and Plantain(INIBAP).Ten out of forty-seven microsatellite primer pairs amplified reproducible and discrete DNA bands,revealing a total of 92 alleles.The average number of alleles amplified by per primer was 9.2,ranging from 5 to 15.The sizes of PCR products were between 75 bp and 310 bp.The genetic similarity among the cultivars was estimated by the Jaccard coefficient,and the similarity value was between 0.1 and 1.Relationships among the cultivars were evaluated using the unweighted pair-grouping with arithmetic average(UPGMA) clustering.The dendrogram showed that the introduced accessions presented higher levels of genetic diversity than the main cultivars.All the cultivars tested could not be discriminated by the 10 primers.