Semiconductor quantum dots (QDs) are nanometre-scale crystals, which have unique photophysical properties, such as size-dependent optical properties, high fluorescence quantum yields, and excellent stability against photobleaching. These properties enable QDs as the promising optical labels for the biological applications, such as multiplexed analysis of immunocomplexes or DNA hybridization processes, cell sorting and tracing, in vivo imaging and diagnostics in biomedicine. Meanwhile, QDs can be used as labels for the electrochemical detection of DNA or proteins. This article reviews the synthesis and toxicity of QDs and their optical and electrochemical bioanalytical applications. Especially the application of QDs in biomedicine such as delivering, cell targeting and imaging for cancer research, and in vivo photodynamic therapy (PDT) of cancer are briefly discussed.
Chan, W.C.; Nie, S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 1998, 281, 2016–2018, doi:10.1126/science.281.5385.2016. 9748158
[3]
Chan, W.C.; Maxwell, D.J.; Gao, X.H.; Bailey, R.E.; Han, M.Y.; Nie, S. Luminescent quantum dots for multiplexed biological detection and imaging. Curr. Opin. Biotechnol 2002, 13, 40–46, doi:10.1016/S0958-1669(02)00282-3. 11849956
[4]
Dabbousi, B.O.; Rodriguez-Viejo, J.; Mikulec, F.V.; Heine, J.R.; Mattoussi, H.; Ober, R.; Jensen, K.F.; Bawendi, M.G. (CdSe) ZnS core-shell quantum dots: Synthesis and characterization of a size series of highly luminescent nanocrystallites. J. Phys. Chem 1997, 101, 9463–9475.
[5]
Lim, Y.T.; Kim, S.; Nakayama, A.; Stott, N.E.; Bawendi, M.G.; Frangioni, J.V. Selection of quantum dot wavelengths for biomedical assays and imaging. Mol. Imaging 2003, 2, 50–64, doi:10.1162/153535003765276282. 12926237
[6]
Mattoussi, H.; Kuno, M.K.; Goldman, E.R.; George, P.; Mauro, J.M. Colloidal semiconductor quantum dot conjugates in biosensing. In Optical Biosensors: Present and Future; Ligler, F.S., Rowe Taitt, C.A., Eds.; Elsevier: Amsterdam, The Netherlands, 2002. Chapter 17; pp. 537–569.
[7]
Michalet, X.; Pinaud, F.F.; Bentolila, L.A. Quantum dots for live cells, in vivo imaging, and diagnostics. Science 2005, 307, 538–544, doi:10.1126/science.1104274. 15681376
[8]
Kim, S.; Lim, Y.T.; Soltesz, E.G.; Grand, A.M.; Lee, J.; Nakayama, A. Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping. Nat. Biotechnol 2004, 22, 93–97, doi:10.1038/nbt920. 14661026
[9]
Chen, F.Q.; Gerion, D. Fluorescent CdSe/ZnS nanocrystal-peptide conjugates for long-term, nontoxic imaging and nuclear targeting in living cells. Nano Lett 2004, 4, 1827–1832, doi:10.1021/nl049170q.
[10]
Somers, R.C.; Bawendi, M.G.; Nocera, D.G. Nanocrystals as sensors. Green Chem 2007, 9, 403–410, doi:10.1039/b706012m.
Smith, A.M.; Duan, H.; Mohs, A.M.; Nie, S. Bioconjugated quantum dots for in vivo molecular and cellular imaging. Adv. Drug Deliv. Rev 2008, 60, 1226–1240, doi:10.1016/j.addr.2008.03.015. 18495291
[13]
Medintz, I.L.; Uyeda, H.T.; Goldman, E.R.; Mattoussi, H. Quantum dot bioconjugates for imaging, labelling and sensing. Nat. Mater 2005, 4, 435–446, doi:10.1038/nmat1390. 15928695
[14]
Jaiswal, J.K.; Mattoussi, H.; Mauro, J.M.; Simon, S.M. Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat. Biotechnol 2003, 21, 47–51, doi:10.1038/nbt767. 12459736
Baron, R.; Willner, B.; Willner, I. Biomolecule-nanoparticle hybrids as functional units for nanobiotechnology. Chem. Commun 2007, 18, 323–332.
[17]
Biju, V.; Muraleedharan, D.; Nakayama, K.; Shinohara, Y.; Itoh, T.; Baba, Y.; Ishikawa, M. Quantum dot-insect neuropeptide conjugates for fluorescence imaging, transfection, and nucleus targeting of living cells. Langmuir 2007, 23, 10254–10261, doi:10.1021/la7012705. 17718524
[18]
Juzenas, P.; Chen, W.; Sun, Y.P.; Coelho, M.; Generalov, R.; Generalova, N.; Christensen, I.L. Quantum dots and nanoparticles for photodynamic and radiation therapy of cancer. Adv. Drug Deliv. Rev 2008, 60, 1600–1614, doi:10.1016/j.addr.2008.08.004. 18840487
[19]
Ochsner, M. Photophysical and photobiological processes in the photodynamic therapy of tumours. J. Photochem. Photobiol. B 1997, 39, 1–18. 9210318
[20]
Oleinick, N.L.; Evans, H.H. The photobiology of photodynamic therapy: Cellular targets and mechanisms. Radiat. Res 1998, 150, 146–156, doi:10.2307/3579816.
[21]
Tardivo, J.P.; Giglio, A.D.; Oliveira, C.S.; Gabrielli, D.S.; Junqueira, H.C.; Tada, D.B.; Severino, D.; Turchiello, R.F.; Baptista, M.S. Methylene blue in photodynamic therapy: From basic mechanisms to clinical applications. Photodiagn. Photodyn. Ther 2005, 2, 175–191, doi:10.1016/S1572-1000(05)00097-9.
[22]
Jiang, Z.; Li, R.; Todd, N.W.; Stass, S.A.; Jiang, F. Detecting genomic aberrations by fluorescence in situ hybridization with quantum dots-labeled probes. J. Nanosci. Nanotechnol 2007, 7, 4254–4259, doi:10.1166/jnn.2007.920. 18283800
[23]
Burke, R.S.; Pun, S.H. Extracellular barriers to in vivo PEI and PEGylated PEI polyplex-mediated gene delivery to the liver. Bioconjug. Chem 2008, 19, 693–704, doi:10.1021/bc700388u. 18293906
[24]
Delehanty, J.B.; Boeneman, K.; Bradburne, C.E.; Robertson, K.; Medintz, I.L. Quantum dots: A powerful tool for understanding the intricacies of nanoparticle-mediated drug delivery. Expert Opin. Drug Deliv 2009, 6, 1091–1112, doi:10.1517/17425240903167934. 19691443
[25]
Zhao, Y.; Zhao, L.; Zhou, L.; Zhi, Y.; Xu, J.; Wei, Z.; Zhang, K.X.; Ouellette, B.F.; Shen, H. Quantum dot conjugates for targeted silencing of bcr/abl gene by RNA interference in human myelogenous leukemia K562 cells. J. Nanosci. Nanotechnol 2010, 10, 5137–5143, doi:10.1166/jnn.2010.2396. 21125862
[26]
Bolhassani, A.; Safaiyan, S.; Rafati, S. Improvement of different vaccine delivery systems for cancer therapy. Mol. Cancer 2011, doi:10.1186/1476-4598-10-3.
[27]
Nabiev, I.; Mitchell, S.; Davies, A.; Williams, Y.; Kelleher, D.; Moore, R.; Gun’ko, Y.K.; Byrne, S.; Rakovich, Y.P.; Donegan, J.F.; Sukhanova, A.; Conroy, J.; Cottell, D.; Gaponik, N.; Rogach, A.; Volkov, Y. General strategy for designing functionalized magnetic microspheres for different bioapplications. Nano Lett 2007, 7, 3452–3461, doi:10.1021/nl0719832. 17949046
[28]
Lewin, M.; Carlesso, N.; Tung, C.H.; Tang, X.W.; Cory, D.; Scadden, D.T.; Weissleder, R. Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells. Nat. Biotechnol 2000, 18, 410–414, doi:10.1038/74464. 10748521
[29]
Dabbousi, B.O.; Murray, C.B.; Rubner, M.F.; Bawendi, M.G. Langmuir-Blodgett manipulation of size-selected CdSe nanocrystallites. Chem. Mater 1994, 6, 216–219, doi:10.1021/cm00038a020.
[30]
Danek, M.; Jensen, K.F.; Murray, C.B.; Bawendi, M.G. Electrospray organometallic chemical vapor deposition-A novel technique for preparation of II–VI quantum dot composites. Appl. Phys. Lett 1994, 65, 2795–2797, doi:10.1063/1.112568.
[31]
Wu, Z.; Zhao, Y.; Qiu, F.; Li, Y.; Wang, S.; Yang, B.; Chen, L.; Sun, J.; Wang, J. Forming water-soluble CdSe/ZnS QDs using amphiphilic polymers, stearyl methacrylate/methylacrylate copolymers with different hydrophobic moiety ratios and their optical properties and stability. Colloids Surf. A Physicochem. Eng. Asp 2009, 350, 121–129, doi:10.1016/j.colsurfa.2009.09.014.
[32]
Ghasemi, Y.; Peymani, P.; Afifi, S. Quantum dot: Magic nanoparticle for imaging, detection and targeting. Acta Biomed 2009, 80, 156–165. 19848055
[33]
Lee, C.M.; Jang, D.; Cheong, S.J.; Kim, E.M.; Jeong, M.H.; Kim, S.H.; Kim, D.W.; Lim, S.T.; Sohn, M.H.; Jeong, H.J. Surface engineering of quantum dots for in vivo imaging. Nanotechnology 2010, doi:10.1088/0957-4484/21/28/285102.
[34]
Uyeda, H.T.; Medintz, I.L.; Jaiswal, J.K.; Simon, S.M.; Mattoussi, H. Synthesis of compact multidentate ligands to prepare stable hydrophilic quantum dot fluorophores. J. Am. Chem. Soc 2005, 127, 3870–3878, doi:10.1021/ja044031w. 15771523
[35]
Pons, T.; Uyeda, H.T.; Medintz, I.L.; Mattoussi, H. Hydrodynamic dimensions, electrophoretic mobility, and stability of hydrophilic quantum dots. J. Phys. Chem. B 2006, 110, 20308–20316, doi:10.1021/jp065041h. 17034212
[36]
Smith, A.M.; Nie, S. Minimizing the hydrodynamic size of quantum dots with multifunctional multidentate polymer ligands. J. Am. Chem. Soc 2008, 130, 11278–11279, doi:10.1021/ja804306c. 18680294
[37]
Kim, S.; Bawendi, M.G. Oligomeric ligands for luminescent and stable nanocrystal quantum dots. J. Am. Chem. Soc 2003, 125, 14652–14653, doi:10.1021/ja0368094. 14640609
[38]
Stewart, M.H.; Susumu, K.; Mei, B.C.; Medintz, I.L.; Delehanty, J.B.; Blanco-Canosa, J.B.; Dawson, P.E.; Mattoussi, H. Multidentate poly(ethylene glycol) ligands provide colloidal stability to semiconductor and metallic nanocrystals in extreme conditions. J. Am. Chem. Soc 2010, 132, 9804–9813, doi:10.1021/ja102898d. 20578776
[39]
Kong, X.L.; Qi, H.; Zhou, H.X.; Ren, L.L.; Deng, C.Y.; Li, F.R. A novel sensitive immunoassay by nucleic acid barcode dot and its application in the detection of prostate-specific antigen. Clin. Chem. Lab. Med 2010, 48, 279–283. 20001442
[40]
Thangadurai, P.; Balaji, S.; Manoharan, P.T. Surface modification of CdS quantum dots using thiols-structural and photophysical studies. Nanotechnology 2008, 19, 435708:1–435708:8.
[41]
Yuan, Z.; Zhang, A.; Cao, Y.; Yang, J.; Zhu, Y.; Yang, P. Effect of mercaptocarboxylic acids on luminescent properties of CdTe quantum dots. J. Fluoresc 2011, doi:10.1007/s10895-011-0937-9.
[42]
Petkar, K.C.; Chavhan, S.S.; Agatonovik-Kustrin, S.; Sawant, K.K. Nanostructured materials in drug and gene delivery: A review of the state of the art. Crit. Rev. Ther. Drug Carr. Syst 2011, 28, 101–164, doi:10.1615/CritRevTherDrugCarrierSyst.v28.i2.10.
[43]
Zhao, L.; Pang, X.; Adhikary, R.; Petrich, J.W.; Jeffries-El, M.; Lin, Z. Organic-inorganic nanocomposites by placing conjugated polymers in intimate contact with quantum rods. Adv. Mater 2011, 23, 2844–2849, doi:10.1002/adma.201100923. 21590817
Sheng, W.; Kim, S.; Lee, J.; Kim, S.W.; Jensen, K.; Bawendi, M.G. In situ encapsulation of quantum dots into polymer microspheres. Langmuir 2006, 22, 3782–3790, doi:10.1021/la051973l. 16584256
[49]
Bolhassani, A. Potential efficacy of cell-penetrating peptides for nucleic acid and drug delivery in cancer. Biochim. Biophys. Acta 2011, 1816, 232–246. 21840374
[50]
Wang, J.; Xia, J. Preferential binding of a novel polyhistidine peptide dendrimer ligand on quantum dots probed by capillary electrophoresis. Anal. Chem 2011, 83, 6323–6329, doi:10.1021/ac2011922. 21728332
[51]
Li, Y.; Zhou, Y.; Wang, H.Y.; Perrett, S.; Zhao, Y.; Tang, Z.; Nie, G. Chirality of glutathione surface coating affects the cytotoxicity of quantum dots. Angew. Chem. Int. Ed. Engl 2011, 50, 5860–5864, doi:10.1002/anie.201008206. 21567671
[52]
Iyer, G.; Pinaud, F.; Xu, J.; Ebenstein, Y.; Li, J.; Chang, J.; Dahan, M.; Weiss, S. Aromatic aldehyde and hydrazine activated Peptide coated quantum dots for easy bioconjugation and live cell imaging. Bioconjug. Chem 2011, 22, 1006–1011, doi:10.1021/bc100593m. 21553893
[53]
Ranjbarvaziri, S.; Kiani, S.; Akhlaghi, A.; Vosough, A.; Baharvand, H.; Aghdami, N. Quantum dot labeling using positive charged peptides in human hematopoetic and mesenchymal stem cells. Biomaterials 2011, 32, 5195–5205, doi:10.1016/j.biomaterials.2011.04.004. 21549422
Sapsford, K.E.; Granek, J.; Deschamps, J.R.; Boeneman, K.; Blanco-Canosa, J.B.; Dawson, P.E.; Susumu, K.; Stewart, M.H.; Medintz, I.L. Monitoring botulinum neurotoxin a activity with peptide-functionalized quantum dot resonance energy transfer sensors. ACS Nano 2011, 5, 2687–2699, doi:10.1021/nn102997b. 21361387
[56]
Liu, H.Y.; Gao, X. Engineering monovalent quantum dot-antibody bioconjugates with a hybrid gel system. Bioconjug. Chem 2011, 22, 510–517, doi:10.1021/bc200004z. 21348524
[57]
Jin, T.; Tiwari, D.K.; Tanaka, S.; Inouye, Y.; Yoshizawa, K.; Watanabe, T.M. Antibody-protein A conjugated quantum dots for multiplexed imaging of surface receptors in living cells. Mol. Biosyst 2010, 6, 2325–2331, doi:10.1039/c0mb00056f. 20835432
[58]
Das, A.; Sanjayan, G.J.; Kecskés, M.; Yoo, L.; Gao, Z.G.; Jacobson, K.A. Nucleoside conjugates of quantum dots for characterization of G protein-coupled receptors: Strategies for immobilizing A2A adenosine receptor agonists. J. Nanobiotechnol 2010, doi:10.1186/1477-3155-8-11.
[59]
Zhao, Y.; Li, Y.; Song, Y.; Jiang, W.; Wu, Z.; Wang, Y.A.; Sun, J.; Wang, J. Architecture of stable and water-soluble CdSe/ZnS core-shell dendron nanocrystals via ligand exchange. J. Colloid. Interface Sci 2009, 339, 336–343, doi:10.1016/j.jcis.2009.08.009. 19735920
Advincula, R.C. Hybrid organic-inorganic nanomaterials based on polythiophene dendronized nanoparticles. Dalton Trans 2006, 23, 2778–2784. 16751885
[62]
Rogach, A.L.; Nagesha, D.; Ostrander, J.W.; Giersig, M.; Kotov, N.A. “Raisin bun”-type composite spheres of silica and semiconductor nanocrystals. Chem. Mater 2000, 12, 2676–2685, doi:10.1021/cm000244i.
[63]
Samia, A.C.; Dayal, S.; Burda, C. Quantum dot-based energy transfer: Perspectives and potential for applications in photodynamic therapy. Photochem. Photobiol 2006, 82, 617–625, doi:10.1562/2005-05-11-IR-525. 16475871
[64]
Fischer, H.; Liu, L.; Pang, K.S.; Chan, W. Pharmacokinetics of nanoscale quantum dots: In vivo distribution, sequestration, and clearance in the rat. Adv. Funct. Mater 2006, 16, 1299–1305, doi:10.1002/adfm.200500529.
Gao, X.H.; Cui, Y.Y.; Levenson, R.M.; Chung, L.W.; Nie, S.M. In vivo cancer targeting and imaging with semiconductor quantum dots. Nat. Biotechnol 2004, 22, 969–976, doi:10.1038/nbt994. 15258594
[68]
Liu, J.; Stephen, K.L.; Vijay, A.V. Molecular mapping of tumor heterogeneity on clinical tissue specimens with multiplexed quantum dots. ACS Nano 2010, 4, 2755–2765, doi:10.1021/nn100213v. 20377268
[69]
Hardman, R. A toxicologic review of quantum dots: Toxicity depends on physicohemical and environmental factors. Environ. Health Perspect 2006, 114, 165–172, doi:10.1289/ehp.8284. 16451849
[70]
Ciofani, G.; Raffa, V.; Menciass, A.; Cuschieri, A. Cytocompatibility, interactions, and uptake of polyethyleneimine-coated boron nitride nanotubes by living cells: Confirmation of their potential for biomedical applications. Biotechnol. Bioeng 2008, 101, 850–858, doi:10.1002/bit.21952. 18512259
[71]
Derfus, A.M.; Chan, W.C.; Bhatia, S.N. Intracellular delivery of quantum dots for live cell labeling of organelle tracking. Adv. Mater 2004, 16, 961–966, doi:10.1002/adma.200306111.
[72]
Chen, H.; Wang, L.; Yeh, J.; Wu, X.; Cao, Z.; Wang, Y.A.; Zhang, M.; Yang, L.; Mao, H. Reducing non-specific binding and uptake of nanoparticles and improving cell targeting with an antifouling PEO-b-PgammaMPS copolymer coating. Biomaterials 2010, 31, 5397–5407, doi:10.1016/j.biomaterials.2010.03.036. 20398933
Parak, W.J.; Boudreau, R.; le Gros, M.; Gerion, D.; Zanchet, D.; Micheel, C.M.; Williams, S.C.; Alivisatos, A.P.; Larabell, C. Cell motility and metastatic potential studies based on quantum dot imaging of phagokinetic tracks. Adv. Mater 2002, 14, 882–885, doi:10.1002/1521-4095(20020618)14:12<882::AID-ADMA882>3.0.CO;2-Y.
[75]
Thein, M.; Cheng, A.; Khanna, P.; Zhang, C.; Park, E.J.; Ahmed, D.; Goodrich, C.J.; Asphahani, F.; Wu, F.; Smith, N.B.; Dong, C.; Jiang, X.; Zhang, M.; Xu, J. Site-specific sonoporation of human melanoma cells at the cellular level using high lateral-resolution ultrasonic micro-transducer arrays. Biosens. Bioelectron 2011, 27, 25–33, doi:10.1016/j.bios.2011.05.026. 21783355
[76]
Susumu, K.; Uyeda, H.T.; Medintz, I.L.; Pons, T.; Delehanty, J.B.; Mattoussi, H. Design of biotin-functionalized luminescent quantum dots. J. Am. Chem. Soc 2007, 129, 13987–13996, doi:10.1021/ja0749744. 17956097
Lieleg, O.; Lopez-Garcia, M.; Semmrich, C.; Auernheimer, J.; Kessler, H.; Bausch, A.R. Specific integrin labeling in living cells using functionalized nanocrystals. Small 2007, 3, 1560–1565, doi:10.1002/smll.200700148. 17705315
[80]
Smith, B.R.; Cheng, Z.; De, A.; Koh, A.L.; Sinclair, R.; Gambhir, S.S. Real-time intravital imaging of RGD-quantum dot binding to luminal endothelium in mouse tumor neovasculature. Nano Lett 2008, 8, 2599–2606, doi:10.1021/nl080141f. 18386933
[81]
Pan, Y.L.; Cai, J.Y.; Qin, L.; Wang, H. Atomic force microscopy-based cell nanostructure for ligand-conjugated quantum dot endocytosis. Acta Biochim. Biophys. Sin 2006, 38, 646–652, doi:10.1111/j.1745-7270.2006.00211.x. 16953304
[82]
Yong, K.T.; Qian, J.; Roy, I.; Lee, H.H.; Bergey, E.J.; Tramposch, K.M.; He, S.; Swihart, M.T.; Maitra, A.; Prasad, P.N. Quantum rod bioconjugates as targeted probes for confocal and two-photon fluorescence imaging of cancer cells. Nano Lett 2007, 7, 761–765, doi:10.1021/nl063031m. 17288490
[83]
Zhang, H.; Sachdev, D.; Wang, C.; Hubel, A.; Gaillard-Kelly, M.; Yee, D. Detection and downregulation of type I IGF receptor expression by antibody-conjugated quantum dots in breast cancer cells. Breast Cancer Res. Treat 2009, 114, 277–285, doi:10.1007/s10549-008-0014-5. 18418709
[84]
Lidke, D.S.; Nagy, P.; Heintzmann, R.; Arndt-Jovin, D.J.; Post, J.N.; Grecco, H.E.; Jares-Erijman, E.A.; Jovin, T.M. Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction. Nat. Biotechnol 2004, 22, 198–203, doi:10.1038/nbt929. 14704683
Diagaradjane, P.; Orenstein-Cardona, J.M.; Colón-Casasnovas, N.E.; Deorukhkar, A.; Shentu, S.; Kuno, N.; Schwartz, D.L.; Gelovani, J.G.; Krishnan, S. Imaging epidermal growth factor receptor expression in vivo: Pharmacokinetic and biodistribution characterization of a bioconjugated quantum dot nanoprobe. Clin. Cancer Res 2008, 14, 731–741, doi:10.1158/1078-0432.CCR-07-1958. 18245533
[87]
Dudu, V.; Rotari, V.; Vazquez, M. Targeted extracellular nanoparticles enable intracellular detection of activated epidermal growth factor receptor in living brain cancer cells. Nanomedicine 2011, 7, 896–903, doi:10.1016/j.nano.2011.05.002. 21683807
[88]
Chakraborty, S.K.; Fitzpatrick, J.A.; Phillippi, J.A.; Andreko, S.; Waggoner, A.S.; Bruchez, M.P.; Ballou, B. Cholera toxin B conjugated quantum dots for live cell labeling. Nano Lett 2007, 7, 2618–2626, doi:10.1021/nl0709930. 17663586
[89]
Jaiswal, J.K.; Goldman, E.R.; Mattoussi, H.; Simon, S.M. Use of quantum dots for live cell imaging. Nat. Methods 2004, 1, 73–78, doi:10.1038/nmeth1004-73. 16138413
[90]
Rajan, S.S.; Liu, H.Y.; Vu, T.Q. Ligand-bound quantum dot probes for studying the molecular scale dynamics of receptor endocytic trafficking in live cells. ACS Nano 2008, 2, 1153–1166, doi:10.1021/nn700399e. 19206333
[91]
Rajan, S.S.; Vu, T.Q. Quantum dots monitor TrkA receptor dynamics in the interior of neural PC12 cells. Nano Lett 2006, 6, 2049–2059, doi:10.1021/nl0612650. 16968024
[92]
Schroeder, J.E.; Shweky, I.; Shmeeda, H.; Banin, U.; Gabizon, A. Folate-mediated tumor cell uptake of quantum dots entrapped in lipid nanoparticles. J. Control. Release 2007, 124, 28–34, doi:10.1016/j.jconrel.2007.08.028. 17928088
[93]
Dudu, V.; Ramcharan, M.; Gilchrist, M.L.; Holland, E.C.; Vazquez, M. Liposome delivery of quantum dots to the cytosol of live cells. J. Nanosci. Nanotechnol 2008, 8, 2293–2300, doi:10.1166/jnn.2008.185. 18572640
[94]
Voura, E.B.; Jaiswal, J.K.; Mattoussi, H.; Simon, S.M. Tracking metastatic tumor cell extravasation with quantum dot nanocrystals and fluorescence emission-scanning microscopy. Nat. Med 2004, 10, 993–998, doi:10.1038/nm1096. 15334072
[95]
Duan, H.; Nie, S. Cell-penetrating quantum dots based on multivalent and endosome-disrupting surface coatings. J. Am. Chem. Soc 2007, 129, 3333–3338, doi:10.1021/ja068158s. 17319667
[96]
Fuente, J.M.; Fandel, M.; Berry, C.C.; Riehle, M.; Cronin, L.; Aitchison, G.; Curtis, A.S. Quantum dots protected with tiopronin: A new fluorescence system for cell-biology studies. ChemBioChem 2005, 6, 989–991, doi:10.1002/cbic.200500071. 15852337
[97]
Farias, PM.; Santos, B.S.; Menezes, F.D. Core-shell CdS/Cd(OH)2 quantum dots: Synthesis and bioconjugation to target red cells antigens. J. Microsc 2005, 219, 103–108, doi:10.1111/j.1365-2818.2005.01501.x. 16176249
[98]
Coulon, J.; Thouvenin, I.; Aldeek, F.; Balan, L.; Schneider, R. Glycosylated quantum dots for the selective labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains. J. Fluoresc 2010, 20, 591–597, doi:10.1007/s10895-009-0590-8. 20058182
[99]
Bharali, D.J.; Lucey, D.W.; Jayakumar, H.; Pudavar, H.E.; Prasad, P.N. Folate-receptor-mediated delivery of InP quantum dots for bioimaging using confocal and two-photon microscopy. J. Am. Chem. Soc 2005, 127, 11364–11371, doi:10.1021/ja051455x. 16089466
[100]
Clarke, S.; Nadeau, J.; Bahcheli, D.; Zhang, Z.; Hollmann, C. Quantum dots as phototoxic drugs and sensors of specific metabolic processes in living cells. Proceedings of the 27th Annual International Conference of the Engineering in Medicine and Biology Society, Shanghai, China, 17–18 January 2005. Volume 1; pp. 504–507.
[101]
Xue, X.; Pan, J.; Xie, H.; Wang, J.; Zhang, S. Fluorescence detection of total count of Escherichia coli and Staphylococcus aureus on water-soluble CdSe quantum dots coupled with bacteria. Talanta 2009, 77, 1808–1813, doi:10.1016/j.talanta.2008.10.025. 19159803
[102]
Clarke, S.J.; Hollmann, C.A.; Zhang, Z.J.; Suffern, D.; Bradforth, S.E.; Dimitrijevic, N.M.; Minarik, W.G.; Nadeau, J.L. Photophysics of dopamine-modified quantum dots and effects on biological systems. Nat. Mater 2006, 5, 409–417, doi:10.1038/nmat1631. 16617348
[103]
Dubertret, B.; Skourides, P.; Norris, D.J.; Noireaux, V.; Brivanlou, A.H.; Libchaber, A. In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science 2002, 298, 1759–1762, doi:10.1126/science.1077194. 12459582
Cai, W.B.; Shin, D.W.; Chen, K.; Gheysens, O.; Cao, Q.Z.; Wang, S.X. Peptide-labeled nearinfrared quantum dots for imaging tumor vasculature in living subjects. Nano Lett 2006, 6, 669–676, doi:10.1021/nl052405t. 16608262
[106]
Yu, X.; Chen, L.; Li, K.; Li, Y.; Xiao, S.; Luo, X. Immunofluorescence detection with quantum dot bioconjugates for hepatoma in vivo. J. Biomed. Opt 2007, 12, 1–5.
[107]
Weng, K.C.; Noble, C.O.; Papahadjopoulos-Sterberg, B.; Chen, F.F.; Drummond, D.C.; Kirpotin, D.B. Targeted tumor cell internalization and imaging of multifunctional quantum dot-conjugated immunoliposomes in vitro and in vivo. Nano Lett 2008, 8, 2851–2857, doi:10.1021/nl801488u. 18712930
[108]
Janib, S.M.; Moses, A.S.; MacKay, J.A. Imaging and drug delivery using theranostic nanoparticles. Adv. Drug Deliv. Rev 2010, 62, 1052–1063, doi:10.1016/j.addr.2010.08.004. 20709124
[109]
Matsuno, A.; Mizutani, A.; Okinaga, H.; Takano, K.; Yamada, S.; Yamada, S.M.; Nakaguchi, H.; Hoya, K.; Murakami, M.; Takeuchi, M.; Sugaya, M.; Itoh, J.; Takekoshi, S.; Osamura, R.Y. Molecular morphology of pituitary cells, from conventional immunohistochemistry to fluorescein imaging. Molecules 2011, 16, 3618–3635, doi:10.3390/molecules16053618. 21540793
