In situ Localization of the MAPK3 Gene Induced by Brown Planthopper in Rice Sheath
褐飞虱取食诱导的MAPK3基因在水稻叶鞘组织中的定位

Northern blot was used to analyze the expression of MAPK3 gene (BPHiw103), and in situ hybridization technique was developed for localization of MAPK3 mRNA in rice sheath fed by brown planthopper (BPH) after48 h. The results revealed that the expression of MAPK mRNA was on the whole up-regulated after BPH feeding. In situ hybridization indicated that it was mostly expressed in parenchyma before BPH feeding, while fed by BPH, this gene was mainly increased in phloem, but down-regulated in parenchyma. Such trend was obviouslydemonstrated in the tissue of inner leaves. All the results showed that the MAPK was induced by BPH feeding, and the gene accumulated in injured rice tissue suggested that it might play the role in the resistant response of rice to BPH feeding.