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Toxins  2010 

Sensing the Deadliest Toxin: Technologies for Botulinum Neurotoxin Detection

DOI: 10.3390/toxins2010024

Keywords: botulinum neurotoxin, detection, endopeptidase, botulism, mouse lethality assay, ELISA, lateral flow test, mass spectrometry, FRET, immuno-PCR

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Abstract:

Sensitive and rapid detection of botulinum neurotoxins (BoNTs), the most poisonous substances known to date, is essential for studies of medical applications of BoNTs and detection of poisoned food, as well as for response to potential bioterrorist threats. Currently, the most common method of BoNT detection is the mouse bioassay. While this assay is sensitive, it is slow, quite expensive, has limited throughput and requires sacrificing animals. Herein, we discuss and compare recently developed alternative in vitro detection methods and assess their ability to supplement or replace the mouse bioassay in the analysis of complex matrix samples.

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