Fibrinogen can transform fibrin through an agglutination reaction, finally forming fibrin polymer with grid structure. The density and viscosity of the reaction system changes drastically during the course of agglutination. In this research, we apply an independently-developed piezoelectric agglutination sensor to detect the fibrinogen agglutination reaction in patients with coronary heart diseases. The terminal judgment method of determining plasma agglutination reaction through piezoelectric agglutination sensor was established. In addition, the standard curve between plasma agglutination time and fibrinogen concentration was established to determinate fibrinogen content quantitatively. The results indicate the close correlation between the STAGO paramagnetic particle method and the method of piezoelectric agglutination sensor for the detection of Fibrinogen. The correlation coefficient was 0.91 (γ = 0.91). The determination can be completed within 10 minutes. The fibrinogen concentration in the coronary heart disease group was significantly higher than that of the healthy control group (P < 0.05). The results reveal that high fibrinogen concentration is closely correlated to the incurrence, development and prognosis of coronary heart diseases. Compared with other traditional methods, the method of piezoelectric agglutination sensor has some merits such as operation convenience, small size, low cost, quick detecting, good precision and the common reacting agents with paramagnetic particle method.
References
[1]
Liu, Z.L.; Ukomadu, C. Fibrinogen-like protein 1, a hepatocyte derived protein is an acute phase reactant. Biochem. Biophys. Res. Commun？2008, 365, 729–734.
[2]
Rijken, D.C.; Dirkx, S.P.G.; Luider, T.M.; Leebeek, F.W.G. Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot. Biochem. Biophys. Res. Commun？2006, 350, 191–194.
[3]
Smith, F.B.; Connor, J.M.; Lee, A.J.; Cooke, A.; Lowe, G.D.O.; Rumley, A.; Fowkes, F.G. Relationship of the platelet glycoprotein PlA and fibrinogen T/G+1689 polymorphisms with peripheral arterial disease and ischaemic heart disease. Thromb. Res？2003, 112, 209–216.
[4]
Adler, G.; Eichman, W.; Szczepanski, M.; Targonska, I.; Jasinska, A. Postoperative plasma interleukin-6 in patients with renal cancer correlates with c-reactive protein but not with total fibrinogen or with high molecular weight fibrinogen fraction. Thromb. Res？1998, 89, 243–248.
[5]
Paraskevas, K.I.; Baker, D.M.; Vrentzos, G.E.; Mikhailidis, D.P. The role of fibrinogen and fibrinolysis in peripheral arterial disease. Thromb. Res？2008, 122, 1–12.
[6]
Pieters, M.; van Zyl, D.G.; Rheeder, P.; Jerling, J.C.; Loots, D.T.; van der Westhuizen, F.H.; Gottsche, L.T.; Weisel, J.W. Glycation of fibrinogen in uncontrolled diabetic patients and the effects of glycaemic control on fibrinogen glycation. Thromb. Res？2007, 120, 439–446.
[7]
Yang, Q.; Tofler, G.H.; Cupples, L.A.; Larson, M.G.; Feng, D.L.; Lindpaintner, K.; Levy, D.; D’Agostino, R.B.; O’Donnell, C.J. A genome-wide search for genes affecting circulating fibrinogen levels in the Framingham Heart Study. Thromb. Res？2003, 110, 57–64.
[8]
Turaj, W.; S？owik, A.; Dziedzic, T.; Pu？yk, R.; Adamski, M.; Strojny, J.; Szczudlik, A. Increased plasma fibrinogen predicts one-year mortality in patients with acute ischemic stroke. J. Neurol. Sci？2006, 246, 13–19.
[9]
Andradesa, M.E.; Lorenzi, R.; Berger, M.; Guimar？es, J.A.; Moreira, J.C.F.; Dal-Pizzol, F. Glycolaldehyde induces fibrinogen post-translational modification, delay in clotting and resistance to enzymatic digestion. Chem.–Bio. Inter？2009, 180, 478–484.
[10]
Carroll, R.C.; Craft, R.M.; Chavez, J.J.; Snider, C.C.; Kirby, R.K.; Cohen, E. Measurement of functional fibrinogen levels using the Thrombelastograph. J. Clin. Anesth？2008, 20, 186–190.
[11]
Elvin, C.M.; Brownlee, A.G.; Huson, M.G.; Tebb, T.A.; Kim, M.; Lyons, R.E.; Vuocolo, T.; Liyou, N.E.; Hughes, T.C.; Ramshaw, J.A.M.; Werkmeister, J.A. The development of photochemically crosslinked native fibrinogen as a rapidly formed and mechanically strong surgical tissue sealant. Biomaterials？2009, 30, 2059–2065.
[12]
Michelsen, A.E.; Santi, C.; Holme, R.; Lord, S.T.; Simpson-Haidaris, P.J.; Solum, N.O.; Pedersen, T.M.; Brosstad, F. The Charge-heterogeneity of Human Fibrinogen as Investigated by 2D Electrophoresis. Thromb. Res？2000, 100, 529–535.
[13]
Yamazaki, M.; Uchiyama, S.; Iwata, M. Measurement of Platelet Fibrinogen Binding and P-Selectin Expression by Flow Cytometry in Patients with Cerebral Infarction. Thromb. Res？2001, 104, 197–205.
[14]
Bach-Gansmo, E.T.; Godal, H.C.; Skjonsberg, O.H. Degradation of Fibrinogen and Cross-Linked Fibrin by Human Neutrophil Elastase Generates D-Like Fragments Detected by ELISA but Not Latex D-Dimer Test. Thromb. Res？1998, 92, 125–134.
[15]
DeAnglis, A.P.; Einhaus, C.M.; Sombun, A.D.; Ee, L.C.; Retzinger, G.S. Retzinger. Fibrinogen in rat gastrointestinal lymph before, during and after intraduodenal administration of emulsified triglyceride: Fibrinogen bound to chylomicrons in gastrointestinal lymph is functional. Thromb. Res？2002, 105, 419–432.
[16]
Chang, S.M.; Muramatsu, H.; Nakamura, C.; Miyake, J. The principle and applications of piezoelectric crystal sensors. Mater. Sci. Eng. C？2000, 12, 111–123.
[17]
O’Kennedy, R.; Leonard, P.; Hearty, S.; Daly, S.; Dillon, P.; Brennan, J.; Dunne, L.; Darmaninsheehan, A.; Stapleton, S.; Tully, E.; Quinn, J.; Chakraborty, T. Advances in biosensors for detection of pathogens in food and water. Enzyme Microb. Technol？2003, 32, 3–13.
[18]
Krim, J. QCM tribology studies of thin adsorbed films. Nano Today？2007, 2, 38–43.
[19]
Kang, Q.; Zhang, H.J.; Liu, X.Y.; Shen, D.Z. Oscillating frequency response of a langasite crystal microbalance in liquid phases. Sens. Actuat. B？2006, 119, 99–104.
[20]
Lin, T.M.; Cheng, T.J.; Wu, T.H.; Chang, H.C. Comparing a piezoelectric quartz crystal with an optical coagulometer in monitoring high-dose heparin therapy by determining whole blood activated partial thromboplastin time and activated clotting time. Sens. Actuat. B？2005, 109, 270–277.
[21]
Sauerbrey, G. Use of vibrating quartz for thin film weighing and microweighing. Z. Phy？1959, 155, 206–222.
[22]
Cheng, T.J.; Lin, T.M.; Wu, T.H.; Chang, H.C. Determination of heparin levels in blood with activated partial thromboplastin time by a piezoelectric quartz crystal sensor. Anal. Chim. Acta？2001, 432, 101–111.
[23]
Scheller, F.W.; Wollenberger, U.; Warsinke, A.; Lisdat, F. Research and development in biosensors. Curr. Opin. Biotechnol？2001, 12, 35–40.
[24]
Wilson, T.L.; Campbell, G.A.; Mutharasan, R. Viscosity and density values from excitation level response of piezoelectric-excited cantilever sensors. Sens. Actuat. A？2007, 138, 44–51.
[25]
Hamsten, A. The hemostatic system and coronary heart disease. Thromb Res？1993, 70, 12–38.
[26]
de Maat, M.P.; Pietersma, A.; Kofflard, M.; Sluiter, W.; Kluft, C. Association of plasma fibrinogen levels with coronary artery disease, smoking and inflammatory markers. Atherosclerosis？1996, 121, 185–191.
