Interaction between PII and NifA in Azospirillum brasilense Sp7
Interaction between PⅡ and NifA in Azospirillum brasilense Sp7

The interaction between PinII and NifA in A. brasilense Sp7 was investigated by using the yeast two-hybrid system. Our experimental results showed that PinII directly interacted with the entire NifA protein and its N-terminal domain, but did not interact with the central domain and the C-terminal domain of NifA. No interaction happened if glnB coding for PinII was frame-shift mutated. Pz, a homolog of PinII, had no interation with NifA.