Carbon monoxide-induced adventitious rooting of hypo-cotyl cuttings from mung bean seedling
Carbon monoxide-induced adventitious rooting of hypocotyl cuttings from mung bean seedling

In this paper, the regulatory role of ex-ogenous carbon monoxide (CO) during the genera-tion and development of adventitious roots in mung bean (Phaseolus radiatus L. cv. Mingguang) hypo-cotyl cuttings was surveyed. The results indicated that, like nitric oxide (NO), CO donor Hematin in-duced adventitious rooting in dose- and time-de- pendent manners. These responses were also prov- en by the addition of gaseous CO aqueous solution with different concentrations. Additionally, the above CO-mediated effect was differently reversed when CO scavenger hemoglobin (Hb), CO specific syn-thetic inhibitor ZnPPIX, the auxin transport inhibitor NPA and nitric oxide synthase (NOS) inhibitor L-NAME were added with Hematin, respectively. Furthermore, after being treated with CO for 36 h, NO fluorescence was significantly enhanced and mainly distributed in the zone of vascular bundles whereas the specific NO scavenger cPTIO significantly inhib-ited NO fluorescence induced by CO, indicating that CO induces adventitious rooting of hypocotyl cuttings from mung bean seedling probably mediated by NO/NOS pathway.