Genetic analysis and mapping of rice (Oryza sativa L.) male-sterile (OsMS-L) mutant
Genetic analysis and mapping of rice （Oryza sativa L.） male-sterile （OsMS-L） mutant

A rice male-sterile mutantOsMS-L ofjaponica cultivar 9522 background, was obtained in M4 population treated with60Co γ-Ray. Genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive gene. Results of tissue section showed that at microspore stage,OsMS-L tapetum was retarded. Then tapetal cells expanded and microspores degenerated. No matured pollens were observed inOsMS-L anther locus. To mapOsMS-L locus, an F2 population was constructed from the cross between theOsMS-L (japonica) and LongTeFu B(indica). Firstly, theOsMS-L locus was roughly mapped between two SSR markers, RM109 and RM7562 on chromosome 2. And then eleven polymorphic markers were developed for further fine fine-mapping. At last theOsMS-L locus was mapped between the two InDel markers, Lhs10 and Lhs6 with genetic distance of 0.4 cM, respectively. The region was delimited to 133 kb. All these results were useful for further cloning and functional analysis ofOsMS-L.