Genetic diversity of Ophiocordyceps sinensis in Gansu Province based on inter-simple sequence repeat (ISSR) analyses
甘肃省冬虫夏草遗传多样性的ISSR分析

Inter-simple sequence repeats has been used in analyzing 18 Ophiocordyceps sinensis samples from 6 areas in Gansu Province. For 15 ISSR primers, each individual showed about 95 DNA bands which ranging in size from 300 to 3,000bp. Molecular phylogenetic tree constructed by genetic similarity coefficient (GS), un-weighted pair group method with arithmetic meanscluster analysis (UPGMA) suggested that the samples isolated from different parts of the same caterpillar have no significant genetic differences; the genetic differentiation of the samples from the same location is not evident while that from different areas is comparatively evident. O. sinensis samples from 6 localities are obviously divided into 3 clustering groups and in the same clustering group there are independent local types. ISSR analysis reveals that O. sinensis shows its genetic variation correlated to its living enviroments.