|
菌物学报 2011
Determination of trehalose in Cordyceps militaris fruiting body
|
Abstract:
In order to determine the content of trehalose in mushroom,a procedure of trehalose extraction from Cordyceps militaris was developed.The results proved that the optimal method for extraction of trehalose was as follows: 1g of powder of Cordyceps militaris fruiting bodies was extracted by reflux in 100mL 90% ethanol for 1h.Then a high performance liquid chromatography (HPLC) was developed for the determination of trehalose.By use of SUGAR SP0810 column (300mm?8mm) as the chromatographic column and purified ...