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菌物学报  2011 

Determination of trehalose in Cordyceps militaris fruiting body
蛹虫草子实体中海藻糖含量的测定

Keywords: edible and medicinal fungi,HPLC,trehalose
食药用真菌,高效液相色谱,海藻糖

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Abstract:

In order to determine the content of trehalose in mushroom,a procedure of trehalose extraction from Cordyceps militaris was developed.The results proved that the optimal method for extraction of trehalose was as follows: 1g of powder of Cordyceps militaris fruiting bodies was extracted by reflux in 100mL 90% ethanol for 1h.Then a high performance liquid chromatography (HPLC) was developed for the determination of trehalose.By use of SUGAR SP0810 column (300mm?8mm) as the chromatographic column and purified ...

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