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菌物学报  2011 

Evaluation of ITS region as a possible DNA barcode for the genus Lachnum (Helotiales)
ITS作为粒毛盘菌属DNA条形码的探索

Keywords: barcoding gap,intra- and inter-specific variation,PCR and sequencing success rate
条形码间隔区,种内与种间变异,PCR扩增及测序成功率

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Abstract:

The feasibility of ITS as a DNA barcode for the genus Lachnum was investigated. Intra- and inter-specific sequence divergences and easiness of sequence acquisition were considered as important criteria to estimate the barcode marker. The results revealed that ITS showed appropriate intra- and inter-specific variations, was able to separate the investigated 22 species of the genus, and was readily PCR amplified and sequenced. We suggest to use ITS as the potential DNA barcode for the genus.

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