Screening of high-yeild strain and medium optimization for maximum production of cordycepin by Cordyeps militaris
虫草素高产菌株的筛选及不同添加物对虫草素产量的影响研究

In order to screen high-yield strain and determine the best harvest time of cordycepin, 14 Cordyceps militaris strains was tested by shaking flask fermentation. The effect of 8 different kinds of nutrient additives on the dynamics of cordycepin accumulation were compared. The results showed that C. militaris CM001 strain produced the highest amount of cordycepin. The biomass of C. militaris reached the maximum of 20.44mg/mL at the 10th day while the maximum cordycepin content reached 73.81mg/L at the 13th day. Seventy percent of cordycepin was in the fermentation broth. Additions of adenosine, adenine, alanine, glycine, and L-aspartic acid considerably increased the cordycepin production in the submerged culture, especially the addition of 1g/L adenine which increasing the cordycepin production by 7.09-fold than the control.